瘦素(LEP)重组蛋白

瘦素(LEP)重组蛋白
适用生物    Bos taurus; Bovine (Cattle，牛)
瘦素(LEP)重组蛋白  
产品应用    SDS-PAGE; WB; ELISA; IP.    规格    50ug
分子量    18.4kDa    价格    请咨询当地代理商
纯度    > 95%    
来源    原核表达

Recombinant Leptin (LEP)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Bos taurus; Bovine (Cattle)
Product No.	HZA084Bo01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	18.4kDa
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)
Residues	Asp29~Gln160 (Accession # P50595) with two N-terminal Tags, His-tag and T7-tag

Formulation

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

瘦素(LEP)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DD TKTLIKTIVT RINDISHTQS VSSKQRVTGL DFIPGLHPLL SLSKMDQTLA IYQQILTSLP SRNVVQISND LENLRDLLHL LAASKSCPLP QVRALESLES LGVVLEASLY STEVVALSRL QGSLQDMLRQ

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

瘦素(LEP)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

瘦素(LEP)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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