适用生物   Rattus norvegicus (Rat，大鼠)
毒蕈碱型胆碱受体M1(CHRM1)重组蛋白
产品应用   SDS-PAGE; WB; ELISA; IP.   规格   50ug
分子量   13.9kDa   价格   请咨询当地代理商
纯度   > 95%
来源   原核表达

Recombinant Cholinergic Receptor, Muscarinic 1 (CHRM1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Rattus norvegicus (Rat)
Product No.	HZA982Ra02
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	13.9kDa
Predicted isoelectric point	n/a
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)

Subcellular Location	n/a
Residues	Ser356~Cys460 (Accession # P08482) with N-terminal His-Tag
Formulation	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-SLVKE KKAARTLSAI LLAFILTWTP YNIMVLVSTF CKDCVPETLW ELGYWLCYVN STVNPMCYAL CNKAFRDTFR LLLLCRWDKR RWRKIPKRPG SVHRTPSRQC

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
毒蕈碱型胆碱受体M1(CHRM1)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

毒蕈碱型胆碱受体M1(CHRM1)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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