磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白
适用生物   Homo sapiens (Human，人)
磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白
产品应用   SDS-PAGE; WB; ELISA; IP.   规格   50ug
分子量   30.9kDa   价格   请咨询当地代理商
纯度   > 95%
来源   原核表达

Recombinant Glypican 4 (GPC4)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZA998Hu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	30.9kDa
Predicted isoelectric point	5.5
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)

Subcellular Location	Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
Residues	Val261~Ala527 (Accession # O75487) with N-terminal His-Tag
Formulation	Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

SEQUENCES

磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-VTVKPCYNYC SNIMRGCLAN QGDLDFEWNN FIDAMLMVAE RLEGPFNIES VMDPIDVKIS DAIMNMQDNS VQVSQKVFQG CGPPKPLPAG RISRSISESA FSARFRPHHP EERPTTAAGT SLDRLVTDVK EKLKQAKKFW SSLPSNVCND ERMAAGNGNE DDCWNGKGKS RYLFAVTGNG LANQGNNPEV QVDTSKPDIL ILRQIMALRV MTSKMKNAYN GNDVDFFDIS DESSGEGSGS GCEYQQCPSE FDYNATDHAG KSANEKA

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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