磷脂酶A1(PLA1)重组蛋白

磷脂酶A1(PLA1)重组蛋白
适用生物    Homo sapiens (Human，人)
磷脂酶A1(PLA1)重组蛋白 
产品应用    SDS-PAGE; WB; ELISA; IP.    规格    50ug
分子量    17.8kDa    价格    请咨询当地代理商
纯度    > 95%    
来源    原核表达

Recombinant Phospholipase A1 (PLA1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZB151Hu02
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	17.8kDa
Predicted isoelectric point	9.3
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)

Subcellular Location	Secreted.
Residues	Leu309~Lys452 (Accession # Q53H76) with N-terminal His-Tag
Formulation	Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

SEQUENCES

磷脂酶A1(PLA1)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- LV EQGGVKIEPL PKEVKVYLLT TSSAPYCMHH SLVEFHLKEL RNKDTNIEVT FLSSNITSSS KITIPKQQRY GKGIIAHATP QCQINQVKFK FQSSNRVWKK DRTTIIGKFC TALLPVNDRE KMVCLPEPVN LQASVTVSCD LK

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

磷脂酶A1(PLA1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
磷脂酶A1(PLA1)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.

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