肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白

肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白
适用生物    Homo sapiens (Human，人)
肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白
产品应用    SDS-PAGE; WB; ELISA; IP.    规格    50ug
分子量    36.4kDa    价格    请咨询当地代理商
纯度    > 95%    
来源    原核表达

Recombinant Peptidyl Arginine Deiminase Type IV (PADI4)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZF709Hu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	36.4kDa
Predicted isoelectric point	6.0
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)

Subcellular Location	Cytoplasm. Nucleus. Cytoplasmic granule.
Residues	Met1~Pro300 (Accession # Q9UM07) with two N-terminal Tags, His-tag and T7-tag
Formulation	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MAQGTLIRVT PEQPTHAVCV LGTLTQLDIC SSAPEDCTSF SINASPGVVV DIAHGPPAKK KSTGSSTWPL DPGVEVTLTM KVASGSTGDQ KVQISYYGPK TPPVKALLYL TGVEISLCAD ITRTGKVKPT RAVKDQRTWT WGPCGQGAIL LVNCDRDNLE SSAMDCEDDE VLDSEDLQDM SLMTLSTKTP KDFFTNHTLV LHVARSEMDK VRVFQATRGK LSSKCSVVLG PKWPSHYLMV PGGKHNMDFY VEALAFPDTD FPGLITLTIS LLDTSNLELP EAVVFQDSVV FRVAPWIMTP

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
肽基精氨酸脱亚氨酶Ⅳ(PADI4)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.

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