Organism species	Mus musculus (Mouse)
Product No.	HZH757Mu02
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	52.6kDa
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)
Residues	Leu59~Phe508 (Accession # O35082) with N-terminal His-Tag

Formulation

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

可罗索(KL)重组蛋白The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGS-LH DTFPDGFLWA VGSAAYQTEG GWRQHGKGAS IWDTFTHHSG AAPSDSPIVV APSGAPSPPL SSTGDVASDS YNNVYRDTEG LRELGVTHYR FSISWARVLP NGTAGTPNRE GLRYYRRLLE RLRELGVQPV VTLYHWDLPQ RLQDTYGGWA NRALADHFRD YAELCFRHFG GQVKYWITID NPYVVAWHGY ATGRLAPGVR GSSRLGYLVA HNLLLAHAKV WHLYNTSFRP TQGGRVSIAL SSHWINPRRM TDYNIRECQK SLDFVLGWFA KPIFIDGDYP ESMKNNLSSL LPDFTESEKR LIRGTADFFA LSFGPTLSFQ LLDPNMKFRQ LESPNLRQLL SWIDLEYNHP PIFIVENGWF VSGTTKRDDA KYMYYLKKFI METLKAIRLD GVDVIGYTAW SLMDGFEWHR GYSIRRGLFY VDFLSQDKEL LPKSSALFYQ KLIEDNGF

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

可罗索(KL)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
可罗索(KL)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.

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