Diaphanous同源物1(DIAPH1)重组蛋白

Diaphanous同源物1(DIAPH1)重组蛋白
适用生物    Homo sapiens (Human，人)
Diaphanous同源物1(DIAPH1)重组蛋白   
产品应用    SDS-PAGE; WB; ELISA; IP.    规格    50ug
分子量    54.5kDa    价格    请咨询当地代理商
纯度    > 95%   
来源    原核表达

Recombinant Diaphanous Homolog 1 (DIAPH1)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!

Organism species	Homo sapiens (Human)
Product No.	HZJ265Hu01
Source	Prokaryotic expression
Host	E.coli
Purity	> 95%
UOM	50ug
Predicted Molecular Mass	54.5kDa
Predicted isoelectric point	5.7
Applications	SDS-PAGE; WB; ELISA; IP.
Endotoxin Level	<1.0EU per 1µg (determined by the LAL method)

Subcellular Location	Cell membrane. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton.
Residues	Asp389~Pro583 (Accession # O60610) with two N-terminal Tags, His-tag and GST-tag
Formulation	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

SEQUENCES

Diaphanous同源物1(DIAPH1)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-DF NEVFQILLNT VKDSKAEPHF LSILQHLLLV RNDYEARPQY YKLIEECISQ IVLHKNGADP DFKCRHLQIE IEGLIDQMID KTKVEKSEAK AAELEKKLDS ELTARHELQV EMKKMESDFE QKLQDLQGEK DALHSEKQQI ATEKQDLEAE VSQLTGEVAK LTKELEDAKK EMASLSAAAI TVPPSVPSRA PVP

USAGE

Reconstitute in sterile PBS, pH7.2-pH7.4.

STORAGE AND STABILITY

Diaphanous同源物1(DIAPH1)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8^oC for one month. Aliquot and store at -80^oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

About the MARKER (complimentary)

Effective Size Range: 10kDa to 70kDa.
Diaphanous同源物1(DIAPH1)重组蛋白Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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