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支链α-酮酸脱氢酶(BCKDK)重组蛋白

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产品名称: 支链α-酮酸脱氢酶(BCKDK)重组蛋白
产品型号: BCKDK
产品展商: HZbscience
产品文档: 无相关文档

简单介绍

支链α-酮酸脱氢酶(BCKDK)重组蛋白敏性高，高效性，原装进口，吸附均匀、吸附性好，回收利用率高、可靠性强，无效包退包换，各种种属重组蛋白产品齐全，质量可靠。支链α-酮酸脱氢酶(BCKDK)重组蛋白热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。


支链α-酮酸脱氢酶(BCKDK)重组蛋白  的详细介绍
支链α-酮酸脱氢酶(BCKDK)重组蛋白
适用生物    Rattus norvegicus (Rat，大鼠)
支链α-酮酸脱氢酶(BCKDK)重组蛋白
产品应用    SDS-PAGE; WB; ELISA; IP.    规格    50ug
分子量    24.5kDa    价格    请咨询当地代理商
纯度    > 95%  
来源    原核表达
Recombinant Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Rattus norvegicus (Rat)
Product No. HZJ560Ra01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 24.5kDa支链α-酮酸脱氢酶(BCKDK)重组蛋白
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Arg44~Thr223 with two N-terminal Tags, His-tag and T7-tag


Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
支链α-酮酸脱氢酶(BCKDK)重组蛋白The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
RERSKTV TSFYNQSAID VVAEKPSVRL TPTMMLYSGR SQDGSHLLKS GRYLQQELPV RIAHRIKGFR SLPFIIGCNP TILHVHELYI RAFQKLTDFP PIKDQADEAQ YCQLVRQLLD DHKDVVTLLA EGLRESRKHI EDEKLVRYFL DKTLTSRLGI RMLATHHLAL HEDKPDFVGI ICT
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
支链α-酮酸脱氢酶(BCKDK)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

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