来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物无标记物
**原甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体WB, ICC, IHC-P, IHC-F, ELISA
Polyclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: GAPDH, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant GAPDH (Gly2~Ser148) expressed in E.coli. Accession No.: RPB932Hu01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF - GKVKVGVNG FGRIGR LVTR AAFNSGKV DI VAI NDPFI DL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIIS [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against GAPDH. It has been selected for its ability to recognize GAPDH in immunohistochemical staining and western blotting. 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体[ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant GAPDH (Gly2~Ser148) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 甘油醛-3-磷酸脱氢酶(GAPDH)多克隆抗体[ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining on fromalin fixed paraffin- embedded liver tissue Used in Western Blot, Sample: Recombinant GAPDH, Human