来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物肌球蛋白ⅠG(MYO1G)多克隆抗体无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用肌球蛋白ⅠG(MYO1G)多克隆抗体WB, ICC, IHC-P, IHC-F, ELISA
Polyclonal Antibody Antibody Antibody Antibody to Myosin IG (MYO1G) (MYO1G) (MYO1G) (MYO1G) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 6th Edition (Revised in March, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: MYO1G, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 100µg Sample: Recombinant MYO1G, Human [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant MYO1G (Gly769~Pro1016) expressed in E.coli. USCN Accession Accession Accession Accession No.: P93436Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. 肌球蛋白ⅠG(MYO1G)多克隆抗体MGHHHHHHSG SEF- GR DLVWPLPPAV LQPFQDTCHA LFCRWRARQL VKNIPPSDMP QIKAKVAAMG ALQGLRQDWG CRRAWARDYL SSATDNPTAS SLFAQRLKTL QDKDGFGAVL FSSHVRKVNR FHKIRNRALL LTDQHLYKLD PDRQYRVMRA VPLEAVTGLS VTSGGDQLVV LHARGQDDLV VCLHRSRPPL DNRVGELVGV LAAHCQGEGR TLEVRVSDCI PLSHRGVRRL ISVEPRPEQP EPDFRCARGS FTLLWP [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against MYO1G. It has been selected for its ability to recognize MYO1G in immunohistochemical staining and western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant MYO1G (Gly769~Pro1016) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 肌球蛋白ⅠG(MYO1G)多克隆抗体[ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.