来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物无标记物
**原髓鞘碱性蛋白(MBP)多克隆抗体-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用髓鞘碱性蛋白(MBP)多克隆抗体WB, ICC, IHC-P, IHC-F, ELISA
Polyclonal Antibody to Myelin Basic Protein (MBP) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: MBP, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 5mg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant MBP (Val220~Met301) expressed in E.coli. USCN Accession No.: RPA539Hu01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFE RQHM DSPD LGT LEV LFQGP LGSPEF -V VHFFKNIVTP RTPPPSQGKG RG LS LS RFSW GAEGQRPGFG YGGRAS DYKS AHKGFKGV DA QGTLSKIFKL GGRDSRSGSP M [ ANTIBODY SPECIFITY ]髓鞘碱性蛋白(MBP)多克隆抗体 The antibody is a rabbit polyclonal antibody raised against MBP. It has been selected for its ability to recognize MBP in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant MBP (Val220~Met301) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. Used in DAB staining on fromalin fixed paraffin- embedded Brain tissue Used in Western Blot ,Sample: Recombinant MBP,Human [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.髓鞘碱性蛋白(MBP)多克隆抗体