来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物E1A激活基因阻遏子1(CREG1)多克隆抗体无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
E1A激活基因阻遏子1(CREG1)多克隆抗体Polyclonal Antibody to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: CREG1, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant CREG1 (Ser66~Thr196) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPQ264Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against CREG1. It has been selected for its ability to recognize CREG1 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user.E1A激活基因阻遏子1(CREG1)多克隆抗体 [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant CREG1 (Ser66~Thr196) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant CREG1, Human Used in DAB staining on fromalin fixed paraffin- embedded liver tissue PAQ264Hu01 Polyclonal Antibody to Cellular Repressor Of E1A Stimulated Genes 1 (CREG1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) Used in Western Blot, Sample: Mouse Ear Tissue Primary Ab: 1µg/mL Rabbit Anti-Human CREG1 Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)E1A激活基因阻遏子1(CREG1)多克隆抗体