来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物无标记物
**原胶质细胞系来源神经营养因子(GDNF)多克隆抗体-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
胶质细胞系来源神经营养因子(GDNF)多克隆抗体PAA043Hu01 PAA043Hu01 PAA043Hu01 Polyclonal Antibody Antibody Antibody Antibody to Glial Cell Line Derived Derived Derived Derived Neurotrophic Neurotrophic Neurotrophic Neurotrophic Factor (GDNF) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 8th Edition (Revised in Jun, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: GDNF, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin Immunoglobulin Immunoglobulin Immunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] 胶质细胞系来源神经营养因子(GDNF)多克隆抗体Immunogen: Immunogen: Immunogen: Immunogen: Recombinant GDNF (Ser78~Ile211) expressed in E.coli. USCN Accession Accession Accession Accession No.: RPA043Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGS-SPD KQMAVLPRRE RNRQAAAANP ENSRGKGRRG QRGKNRGCVL TAIHLNVTDL GLGYETKEEL IFRYCSGSCD AAETTYDKIL KNLSRNRRLV SDKVGQACCR PIAFDDDLSF LDDNLVYHIL RKHSAKRCGC I [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against GDNF. It has been selected for its ability to recognize GDNF in immunohistochemical staining and western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant GDNF (Ser78~Ile211) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining staining staining staining on fromalin fromalin fromalin fromalin fixed paraffin- paraffin- paraffin- paraffin- embedded embedded embedded embedded Brain tissue Used in Western Western Western Western Blot ,Sample: ,Sample: ,Sample: ,Sample: Recombinant Recombinant Recombinant Recombinant GDNF,Human GDNF,Human GDNF,Human GDNF胶质细胞系来源神经营养因子(GDNF)多克隆抗体