来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
标记物无标记物
**原胰岛素样生长因子结合蛋白1(IGFBP1)多克隆抗体-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
胰岛素样生长因子结合蛋白1(IGFBP1)多克隆抗体Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 1 (IGFBP1) Organism Species: Bos taurus; Bovine (Cattle) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: IGFBP1, Bovine Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg Sample: Recombinant IGFBP1, Bovine [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant IGFBP1 (Asp112~Asn263) expressed in E.coli. USCN Accession No.: RPA052Bo01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-DEATDTKDT TSPENVSPES SEITQEQLLD NFHLMAESSE DLPILWNAIS NYESLRALEI SDVKKWKEPC QRELYKVLDR LA REQQKAGD KLYKFY LP NC NK NG FY HSKQ CETS LEGE PG LCWCVYPWSG KRILGSVAVR GDPKCQQYFN LQN [ ANTIBODY SPECIFITY ] 胰岛素样生长因子结合蛋白1(IGFBP1)多克隆抗体The antibody is a rabbit polyclonal antibody raised against IGFBP1. It has been selected for its ability to recognize IGFBP1 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant IGFBP1 (Asp112~Asn263) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.胰岛素样生长因子结合蛋白1(IGFBP1)多克隆抗体