半乳糖凝集素3(GAL3)多克隆抗体Polyclonal Antibody to Galectin 3 (GAL3) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: GAL3, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant GAL3 (Met1~Ile250) expressed in E.coli. Accession No.: RPA303Hu01 Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGS-MADNFSLHDA LSGSGNPNPQ GWPGAWGNQP AGAGGYPGAS YPGAYPG QAP PGAY PG QA PP GAYPGAP GAY PGA PA PGVYP GPP SGPGAYP SSGQPSATGA YPATGPYGAP AGPLIVPYNL PLPGGVVPRM LITILGTVKP NANRIALDFQ RGNDVAFHFN PRFNENNRRV IVCNTKLDNN WGREERQSVF PFESGKPFKI QVLVEPDHFK VAVNDAHLLQ YNHRVKKLNE ISKLGISGDI DLTSASYTMI [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against GAL3. It has been selected for its ability to recognize GAL3 in immunohistochemical staining and western blotting.半乳糖凝集素3(GAL3)多克隆抗体 [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant GAL3 (Met1~Ile250) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining on fromalin fixed paraffin- embedded Heart tissue Used in Western Blot ,Sample: Recombinant GAL3半乳糖凝集素3(GAL3)多克隆抗体