基质金属蛋白酶15(MMP15)多克隆抗体Polyclonal Antibody to Matrix Metalloproteinase 15 (MMP15) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: MMP15, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant MMP15 (Phe280~Asp497) expressed in E.coli. Accession No.: RPA322Hu01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-F YQWKDVDNFK LP E D D L R GI Q Q LY G TP D G Q P QP TQ P L P T V T P R RP G RP D H R PP RP P QP P P P GGK PE RP PK P GPP VQP RAT E RP DQY GP NI C DG D F D TVAM L RGEM F VF KG R WFW RV RH NRV LD NYPMPIGH FW RG LPGDIS AAYE RQ DG RF VF FKG DRYW L FREANLEPGY PQPLTSYGLG IPYDRIDTAI WWEPTGHTFF FQEDRYWRFN EETQRGD [ ANTIBODY SPECIFITY ] 基质金属蛋白酶15(MMP15)多克隆抗体The antibody is a rabbit polyclonal antibody raised against MMP15. It has been selected for its ability to recognize MMP15 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant MMP15 (Phe280~Asp497) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. Used in DAB staining on fromalin fixed paraffin- embedded liver tissue Used in Western Blot, Sample: Recombinant MMP15, Human [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.基质金属蛋白酶15(MMP15)多克隆抗体