心肌肌钙蛋白I(TNNI3)多克隆抗体Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Polyclonal antibody preparation Host: Rabbit Purification: Antigen-specific Affinity Chromatography. Traits: Liquid Concentration: 200µg/mL UOM: 10µg Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ] Immunogen: RPA478Hu01-Recombinant Troponin I Type 3, Cardiac (TNNI3) [ APPLICATIONS ] Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. [ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 心肌肌钙蛋白I(TNNI3)多克隆抗体[ QUALITY CONTROL ] Content: The quality control contains recombinant TNNI3 disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.心肌肌钙蛋白I(TNNI3)多克隆抗体 [ IDENTIFICATION ] A B Figure 1. Western Blot A. Sample: Recombinant TNNI3, Human B. Lane1: Mouse Heart Tissue Lane2: Mouse Skeletal Muscle Tissue Lane3: Porcine Heart Tissue Lane4: Porcine Skeletal Muscle Tissue Primary Ab: 2µg/mL Rabbit Anti-Human TNNI3 Ab Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SAA544Rb59)