内皮素转化酶1(ECE1)多克隆抗体Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: ECE1, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant ECE1 (Leu469~Glu721) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA483Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against ECE1. It has been selected for its ability to recognize ECE1 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 内皮素转化酶1(ECE1)多克隆抗体[ QUALITY CONTROL ] Content: The quality control contains recombinant ECE1 (Leu469~Glu721) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant ECE1, Human Used in DAB staining on fromalin fixed paraffin- embedded liver cancer tissue PAA483Hu01 Polyclonal Antibody to Endothelin Converting Enzyme 1 (ECE1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in DAB staining on fromalin fixed paraffinembedded prostate glands cancer tissue Used in DAB staining on fromalin fixed paraffin- embedded prostate glands tissue Used in DAB staining on fromalin fixed paraffin- embedded rectum cancer tissue内皮素转化酶1(ECE1)多克隆抗体