纤溶酶原激活物抑制因子1(PAI1)多克隆抗体PAA532Hu01 PAA532Hu01 PAA532Hu01 Polyclonal Antibody Antibody Antibody Antibody to Plasminogen Plasminogen Plasminogen Plasminogen Activator Activator Activator Activator Inhibitor Inhibitor Inhibitor Inhibitor 1 (PAI1) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 8th Edition (Revised in Jun, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: PAI1, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin Immunoglobulin Immunoglobulin Immunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant PAI1 (His25~Pro402) expressed in E.coli. 纤溶酶原激活物抑制因子1(PAI1)多克隆抗体USCN Accession Accession Accession Accession No.: RPA532Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- HHPPSY VAHLASDFGV RVFQQVAQAS KDRNVVFSPY GVASVLAMLQ LTTGGETQQQ IQAAMGFKID DKGMAPALRH LYKELMGPWN KDEISTTDAI FVQRDLKLVQ GFMPHFFRLF RSTVKQVDFS EVERARFIIN DWVKTHTKGM ISNLLGKGAV DQLTRLVLVN ALYFNGQWKT PFPDSSTHRR LFHKSDGSTV SVPMMAQTNK FNYTEFTTPD GHYYDILELP YHGDTLSMFI AAPYEKEVPL SALTNILSAQ LISHWKGNMT RLPRLLVLPK FSLETEVDLR KPLENLGMTD MFRQFQADFT SLSDQEPLHV AQALQKVKIE VNESGTVASS STAVIVSARM APEEIIMDRP FLFVVRHNPT GTVLFMGQVM EP [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against PAI1. It has been selected for its ability to recognize PAI1 in immunohistochemical staining and western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant PAI1 (His25~Pro402) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). 纤溶酶原激活物抑制因子1(PAI1)多克隆抗体Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining staining staining staining on fromalin fromalin fromalin fromalin fixed paraffin- paraffin- paraffin- paraffin- embedded embedded embedded embedded Kidney tissue Used in Western Western Western Western Blot ,Sample: ,Sample: ,Sample: ,Sample: Recombinant Recombinant Recombinant Recombinant PAI1,Human PAI1,Human PAI1,Human PAI1,Human