水通道蛋白1(AQP1)多克隆抗体Polyclonal Antibody to Aquaporin 1, Colton Blood Group (AQP1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: AQP1, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant AQP1 (Val201~Lys269) with two N-terminal Tags, His-tag and GST-tag expressed in E.coli. Accession No.: RPA579Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against AQP1. It has been selected for its ability to recognize AQP1 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. 水通道蛋白1(AQP1)多克隆抗体[ QUALITY CONTROL ] Content: The quality control contains recombinant AQP1 (Val201~Lys269) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant AQP1, Human Used in DAB staining on fromalin fixed paraffin- embedded kidney tissue PAA579Hu01 Polyclonal Antibody to Aquaporin 1, Colton Blood Group (AQP1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in DAB staining on fromalin fixed paraffin- embedded liver cancer tissue Used in DAB staining on fromalin fixed paraffin- embedded liver tissue Used in DAB staining on fromalin fixed paraffin- embedded glioma tissue Used in DAB staining on fromalin fixed paraffinembedded prostate glands cancer tissue PAA579Hu01 Polyclonal Antibody to Aquaporin 1, Colton Blood Group (AQP1) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in DAB staining on fromalin fixed paraffinembedded rectum cancer tissue水通道蛋白1(AQP1)多克隆抗体