CD14分子(CD14)多克隆抗体Polyclonal Antibody to Cluster Of Differentiation 14 (CD14) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: CD14, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant CD14 (Asp125~Leu288) with N-terminal His-Tag expressed in E.coli. Accession No.: RPA685Hu01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against CD14. It has been selected for its ability to recognize CD14 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user.CD14分子(CD14)多克隆抗体[ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant CD14 (Asp125~Leu288) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.CD14分子(CD14)多克隆抗体 [ IMAGES ] Used in Western Blot, Sample: Recombinant CD14, Human