激肽释放酶6(KLK6)多克隆抗体Polyclonal Antibody Antibody Antibody Antibody to Kallikrein Kallikrein Kallikrein Kallikrein 6 (KLK6) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 7th Edition (Revised in May, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: KLK6, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 100µg Sample: Recombinant KLK6, Human [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant KLK6 (Glu17~Lys244) expressed in E.coli. USCN Accession Accession Accession Accession No.: rP90691Hu01 Sequence: Sequence: Sequence: Sequence: 激肽释放酶6(KLK6)多克隆抗体The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GS T S GS G H H H HH HSA G LVP R GS TAI GM KE T AAAK F E R Q HM DSP D LG T LEV LFQGPLGSEF-EEQN KLVHGGPCDK TSHPYQAALY TSGHLLCGGV LIHPLWVLTA AH C K K P N L Q V F L G K H N L R Q R ES S Q E Q S S V V RAV I H P D Y D A AS H D Q D I M L L RLARPAKLSELIQPLPLERD CSANTTSCHI LGWGKTADGD FPDTIQCAYI HLVSREECEH AYPGQITQ NM LCAG DEKYGK DSC QG DS GGP LVC GD H L R G L VSWG NI PCGS KEKPGVYTNV CRYTNWIQKT IQAK [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against KLK6. It has been selected for its ability to recognize KLK6 in immunohistochemical staining and western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant KLK6 (Glu17~Lys244) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.激肽释放酶6(KLK6)多克隆抗体