27kDa热休克蛋白(HSPB1)多克隆抗体Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1) Organism Species: Bos taurus; Bovine (Cattle) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PRODUCT INFORMATION ] Immunogen: HSPB1, Bovine Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant HSPB1 (Met1~Lys201) with two N-terminal Tags, His-tag and T7-tag expressed in E.coli. Accession No.: RPA693Bo01 [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against HSPB1. It has been selected for its ability to recognize HSPB1 in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-5000 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant HSPB1 (Met1~Lys201) disposed in loading buffer.27kDa热休克蛋白(HSPB1)多克隆抗体 Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. [ IMAGES ] Used in Western Blot, Sample: Recombinant HSPB1, Bovine PAA693Bo01 Polyclonal Antibody to Heat Shock 27kDa Protein 1 (HSPB1) Organism Species: Bos taurus; Bovine (Cattle) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES Used in Western Blot, Sample: Lane1: Bovine Serum Lane2: Bovine Heart Tissue Lane3: HeLa Cells Lane4: Bovine Skeletal Muscle Tissue Lane5: Mcf7 Cells Primary Ab: 3µg/mL Rabbit Anti-Bovine HSPB1 Ab Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig Anti-Rabbit Ab (Catalog: SECab05)27kDa热休克蛋白(HSPB1)多克隆抗体