C-型钠尿肽(CNP)多克隆抗体90721Hu01 90721Hu01 90721Hu01 Polyclonal Antibody Antibody Antibody Antibody to C-Type Natriuretic Natriuretic Natriuretic Natriuretic Peptide Peptide Peptide Peptide (CNP) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 5th Edition (Revised in January, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: CNP Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Species Species Species Species Reactivity: eactivity: eactivity: eactivity: Human Ig type: Rabbit IgG Purification: Purification: Purification: Purification: Antibodies are purified by target protein affinity chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Form: Liquid Size: 100µg Sample: Recombinant human CNP [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human CNP (Arg33~Gly125) expressed in E.coli. Molecular Molecular Molecular Molecular Weight: Weight: Weight: Weight: 11.3kDa USCN accession accession accession accession No.: P90721Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. C-型钠尿肽(CNP)多克隆抗体MGHHHHHHSGSEF- RTPPAEEL AEPQAAGGGQ KKGDKAPGGG GANLKGDRSR LLRDLRVDTK SRAAWARLLQ EHPNARKYKG ANKKGLSKGC FGLKLDRIGS MSGLG [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPEFICITY SPEFICITY SPEFICITY SPEFICITY ] Anti CNP is a rabbit polyclonal antibody raised against human CNP. This antibody has been selected for its ability to recognize human CNP in immunohistochemical staining and western blotting, non cross-reactive with other members of the family. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N, 50% glycerol.C-型钠尿肽(CNP)多克隆抗体 [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant human CNP (Arg33~Gly125) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading buffer: buffer: buffer: buffer: 100mM Tris(PH 8.8), 2%SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, Na3N 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.