补体成分7(C7)多克隆抗体 Polyclonal Antibody to Complement Component 7 (C7) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: C7, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200µg/mL UOM: 100µg Sample: Recombinant C7, Human [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant C7 (Ser122~His456) linked with LEHHHHHH expressed in E.coli. Accession No.: RPA731Hu01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SERRPSCDI DKPPPNIELT GNGYNELTGQ FRNRVINTKS FGGQCRKVFS GDGKDFYRLS GNVLSYTFQV KINNDFNYEF YNSTWSYVKH TSTEHTSSSR KRSFFRSSSS SSRSYTSHTN EIHKGKSYQL LVVENTVEVA QFINNNPEFL QLAEPFWKEL SHLPSLYDYS AYRRLIDQYG THYLQSGSLG GEYRVLFYVD SEKLKQNDFN SVEEKKCKSS GWHFVVKFSS HGCKELENAL KAASGTQNNV LRGEPFIRGG GAGFISGLSY LELDNPAGNK RRYSAWAESV TNLPQVIKQK LTPLYELVKE VPCASVKKLY LKWALEEYLD EFDPCH LEHHHHHH [ ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against C7. It has been selected for its ability to recognize C7 in immunohistochemical staining and western blotting. 补体成分7(C7)多克隆抗体[ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant C7 disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 补体成分7(C7)多克隆抗体[ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.