血管紧张素原(AGT)多克隆抗体Antibody Antibody Antibody Antibody to Angiotensinogen Angiotensinogen Angiotensinogen Angiotensinogen (AGT) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 6th Edition (Revised in March, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: AGT Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Organism: Organism: Organism: Organism: Human Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 50µg Sample: Recombinant Human AGT [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human AGT (Ala155~Lys355) expressed in E.coli. USCN Accession Accession Accession Accession No.: P90797Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF-ADRLQA ILGVPWKDKN CTSRLDAHKV LSALQAVQGL LVAQGRADSQ AQLLLSTVVG VFTAPGLHLK QPFVQGLALY TPVVLPRSLD FTELDVAAEK IDRFMQAVTG WKT G CS LMGA SV DS T L AF NT YV HF QGKMK G FS L LAEPQE F WVD NST SVSV PMLSGMGTFQ HWSDIQDNFS VTQVPFTESA CLLLIQPHYA SDLDK血管紧张素原(AGT)多克隆抗体 [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] Anti AGT is a rabbit polyclonal antibody raised against human AGT. This antibody has been selected for its ability to recognize human AGT in immunohistochemical staining and western blotting, non cross-reactive with other members of the family. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% Na3N, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant human AGT(Ala155~Lys355) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, Na3N 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.血管紧张素原(AGT)多克隆抗体