凝血因子Ⅸ(F9)多克隆抗体Polyclonal Antibody Antibody Antibody Antibody to Coagulation Coagulation Coagulation Coagulation Factor IX (F9) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 6th Edition (Revised in March, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: F9, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Concentration: Concentration: Concentration: Concentration: 200µg/mL UOM: 100µg Sample: Recombinant F9, Human [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen : Immunogen : Immunogen : Immunogen : Recombinant F9 (Asp232~Lys455) exp ressed in E.coli. USCN Accession Accession Accession Accession No.: P90840Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSGSEF - DAKPGQFPW QVVLNGKVDA FCGGSIVNEK WIVTAAHCVE TGVKITVVAG EHNIEETEHT EQKRNVIRII PHHNYNAAIN KYNHDIALLE LDEPLVLNSY VTPICIADKE YTNIFLKFGS GYVSGWGRVF HKGRSALVLQ YLRVPLVDRA TCLRSTKFTI YNNM F CAG F H EGG R DSC QG D SGGPHVTEVE GTS F LTGIIS WGEECAMK GK YGIYTKVSRY VNWIK 凝血因子Ⅸ(F9)多克隆抗体[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against F9. It has been selected fo r it s ab i l it y to re c og n i z e F9 in immu n oh i st o ch emi ca l st a i n i n g an d western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant F9 (Asp232~Lys455) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.凝血因子Ⅸ(F9)多克隆抗体