过氧化物酶体增殖物激活受体γ(Pklγ)多克隆抗体
Polyclonal Antibody
To Peroxisome Proliferator Activated Receptor Gamma (PPARg)
Organism Species: Bos taurus; Bovine (Cattle)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: PPARg, Bovine
Clonality: Polyclonal
过氧化物酶体增殖物激活受体γ(Pklγ)多克隆抗体Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant PPARg (Gln311~Ile500) with N-terminal His-Tag
expressed in E.coli.
Accession No.: RPA886Bo01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against PPARg. It has been
selected for its ability to recognize PPARg in immunohistochemical staining and
western blotting.
过氧化物酶体增殖物激活受体γ(Pklγ)多克隆抗体[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant PPARg (Gln311~Ile500)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
BPB 0.01%, NaN3 0.02%.过氧化物酶体增殖物激活受体γ(Pklγ)多克隆抗体
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant PPARg, Bovine
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物无标记物
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**原过氧化物酶体增殖物激活受体γ(PPARg)重组蛋白
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缓冲液成份过氧化物酶体增殖物激活受体γ(Pklγ)多克隆抗体磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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