丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体
Polyclonal Antibody to Pyruvate Dehydrogenase Phosphatase (PDP)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: PDP, Human
Clonality: Polyclonal
丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant PDP (Asn315~Glu537) expressed in E.coli. Accession No.: RPA887Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NAQNER ELERLKLEHP
KSEAKSVVKQ DRLLGLLMPF RAFGDVKFKW SIDLQKRVIE SGPDQLNDNE YTKFIPPNYH
TPPYLTAEPE VTYHRLRPQD KFLVLATDGL WETMHRQDVV RIVGEYLTGM HHQQPIAVGG
YKVTLGQMHG LLTERRTKMS SVFEDQNAAT HLIRHAVGNN EFGTVDHERL SKMLSLPEEL
ARMYRDDITI IVVQFNSHVV GAYQNQE
[ ANTIBODY SPECIFITY ]丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体
The antibody is a rabbit polyclonal antibody raised against PDP. It has been
selected for its ability to recognize PDP in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant PDP (Asn315~Glu537)
disposed in loading buffer.丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Kidney tissue
Used in Western Blot, Sample:
Recombinant PDP, Human
-
来源丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体多抗制备
-
宿主兔
-
效价-
-
Ig类型IgG
-
纯化方式抗原特异性亲和纯化
-
标记物无标记物
-
**原-
-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
-
性状液体
-
浓度200ug/ml
-
且适物种-
-
应用丙酮酸脱氢酶磷酸酶(PDP)多克隆抗体WB, ICC, IHC-P, IHC-F, ELISA
-
