骨桥素(OPN)多克隆抗体
Polyclonal Antibody to Osteopontin (OPN)
Organism Species: Canis familiaris; Canine (Dog)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: OPN, Canine
Clonality: Polyclonal
Host: Rabbit骨桥素(OPN)多克隆抗体
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F
Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant OPN, Canine
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant OPN (Ile7~Ser232) expressed in E.coli. USCN Accession No.: RPA899Ca01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-IAYA IPIKHADSGS SEEKQNAVLT EETDDFKQKT FSSKSNESHD
DVDEDDGDDV DSQDSVDSND LDDDSNESDE SDELVTDFPT DIPATQLFTP AVPTRGSYDG
RGDSVAYGLR SKSKKSHKYE VQYPDSTEED FTSLVKSASM EDDFNAVLLS RTVRGTSDRD
SHAK DS QETS QL D D HSMET K GRK HS QE YK L RA S DES NM HS HEIGSQE NS E
VSSELVSQLS QS
[ ANTIBODY SPECIFITY ]骨桥素(OPN)多克隆抗体
The antibody is a rabbit polyclonal antibody raised against OPN. It has been
selected for its ability to recognize OPN in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant OPN (Ile7~Ser232) disposed
in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.骨桥素(OPN)多克隆抗体
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物骨桥素(OPN)多克隆抗体无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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