胞外5'-核苷酸酶(NT5E)多克隆抗体
Polyclonal Antibody to 5'-Nucleotidase, Ecto (NT5E)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: NT5E, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.胞外5'-核苷酸酶(NT5E)多克隆抗体
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant NT5E (Leu29~Thr500) expressed in E.coli. Accession No.: RPB250Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF-LT ILHTNDVHSR LEQTSEDSSK CVNASRCMGG VARLFTKVQQ
IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE VAHFMNALRY DAMALGNHEF DNGVEGLIEP
LLKEAKFPIL SANIKAKGPL ASQISGLYLP YKVLPVGDEV VGIVGYTSKE TPFLSNPGTN
LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV GGHSNTFLYT
GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV ISSHGNPILL
NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS QSCRFRECNM GNLICDAMIN
NNLRHTDEMF WNHVSMCILN GGGIRSPIDE RNNGIHVVYD LSRKPGDRVV KLDVLCTKCR
VPSYDPLKMD EVYKVILPNF LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP
AVEGRIKFST胞外5'-核苷酸酶(NT5E)多克隆抗体
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against NT5E. It has been
selected for its ability to recognize NT5E in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant NT5E (Leu29~Thr500)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.胞外5'-核苷酸酶(NT5E)多克隆抗体
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin- embedded Liver tissue
Used in Western Blot, Sample:
Recombinant NT5E, Human
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式胞外5'-核苷酸酶(NT5E)多克隆抗体抗原特异性亲和纯化
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标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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