雄**受体(AR)多克隆抗体
Polyclonal Antibody to Androgen Receptor (AR)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
[ PRODUCT INFORMATION ]
Immunogen: AR
Clonality: Polyclonal
Host: Rabbit
Species Reactivity: Human
Ig type: Rabbit IgG
Purification: Antibodies are purified by
target protein affinity chromatography.雄**受体(AR)多克隆抗体
Applications: WB, ICC, IHC-P, IHC-F
Form: Liquid
Size: 100µg
Sample: Recombinant human AR
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant human AR (Pro15~Ser246) expressed in E.coli.
Molecular Weight: 30.5kDa
USCN accession No.: P91252Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- PSKTYR
GAFQNLFQSV REVIQNPGPR HPEAASAAPP GASLLLLQQQ QQQQQQQQQQ
QQQQQQQQQQ ETSPRQQQQQ QGEDGSPQAH RRGPTGYLVL DEEQQPSQPQ
SALECHPERG CVPEPGAAVA ASKGLPQQLP APPDEDDSAA PSTLSLLGPT FPGLSSCSAD
LKDILSEAST MQLLQQQQQE AVSEGSSSGR AREASGAPTS SKDNYLGGTS TISDNAKELC
KAVSVS
[ ANTIBODY SPEFICITY ]雄**受体(AR)多克隆抗体
Anti AR is a rabbit polyclonal antibody raised against human AR. This antibody has
been selected for its ability to recognize human AR in immunohistochemical
staining and western blotting, non cross-reactive with other members of the family.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N,
50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant human AR (Pro15~Ser246)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not
used for other purposes.
Loading buffer: 100mM Tris(PH 8.8), 2%SDS, 200mM NaCl, 50% glycerol, BPB
0.01%, Na3N 0.02%.雄**受体(AR)多克隆抗体
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液(pH7.4,含有0.02% NaN3和50%甘油)
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性状雄**受体(AR)多克隆抗体液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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