轴突生长诱向因子1(Ntn1)多克隆抗体
Polyclonal Antibody Antibody Antibody Antibody to Netrin 1 (Ntn1)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Ntn1, Human
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.轴突生长诱向因子1(Ntn1)多克隆抗体
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Concentration: Concentration: Concentration: Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant Ntn1, Human
[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant Ntn1 (Pro313~Pro565) expressed in E.coli. USCN Accession Accession Accession Accession No.: P91827Hu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tags-Tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ
GPLGSEF- PECDRCKP FHYDRPWQRA TAREANECVA CNCNLHARRC RFNMELYKLS
GRKSGGVC L N CRHNTAGRH C HYCKEGYYRD MGKPIT HRKA CKACDCHPVG
AAGKTCNQTT GQCPCKDGVT GITCNRCAKG YQQSRSPIAP CIKIPVAPPT TAASSVEEPE
DCDSYCKASK GKLKINMKKY CKKDYAVQIH ILKADKAGDW WKFTVNIISV YKQGTSRIRR
GDQSLWIRSR DIACKCPKIK PLKKYLLLGN AEDSP
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]轴突生长诱向因子1(Ntn1)多克隆抗体
The antibody is a rabbit polyclonal antibody raised against Ntn1. It has been
selected for its ability to recognize Ntn1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant Ntn1 (Pro313~Pro565)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.轴突生长诱向因子1(Ntn1)多克隆抗体
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状轴突生长诱向因子1(Ntn1)多克隆抗体液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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