肉毒碱乙酰转移酶(CRAT)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物肉毒碱乙酰转移酶(CRAT)多克隆抗体无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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肉毒碱乙酰转移酶(CRAT)多克隆抗体
Polyclonal Antibody to Carnitine Acetyltransferase (CRAT)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CRAT, Mouse
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.肉毒碱乙酰转移酶(CRAT)多克隆抗体
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CRAT (Met1~Asp430) expressed in E.coli. Accession No.: RPC400Mu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- MLAFAARTVV KPLGLLKPSS
LMKVSGRFKA HQDALPRLPV PPLQQSLDYY LKALQPIVSE EEWAHTKQLV DEFQTSGGVG
ERLQKGLERR AKKMENWLSE WWLKTAYLQF RQPVVIYSSP GVILPKQDFV DLQGQLRFAA
KLIEGVLDFK SMIDNETLPV EFLGGQPLCM NQYYQILSSC RVPGPKQDSV VNFLKSKRPP
THITVVHNYQ FFELDVYHSD GTPLTSDQIF VQLEKIWNSS LQSNKEPVGI LTSNHRNTWA
KAYNNLIKDK VNRESVNSIQ KSIFTVCLDK QVPRVSDDVY RNHVAGQMLH GGGSKFNSGN
RWFDKTLQFI VAEDGSCGMV YEHAAAEGPP IVALVDHVME YTKKPELVRS PMVPLPMPKK
LRFNITPEIK NDIEKAKQNL SIMIQDLDIM MLTFHHFGKD FPKSEKLSPD
[ ANTIBODY SPECIFITY ]肉毒碱乙酰转移酶(CRAT)多克隆抗体
The antibody is a rabbit polyclonal antibody raised against CRAT. It has been
selected for its ability to recognize CRAT in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant CRAT (Met1~Asp430)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.肉毒碱乙酰转移酶(CRAT)多克隆抗体
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded testis tissue
Used in Western Blot, Sample:
Recombinant CRAT, Mouse
