Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
Polyclonal Antibody to Myeloid Cell Leukemia Sequence 1, Bcl2 Related (MCL1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: MCL1, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
Immunogen: Recombinant MCL1 (Arg6~Ile328) expressed in E.coli. Accession No.: RPC615Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF -RNAVI GLNLYCGGAG
LGAGSGGATR PGGRLLATEK EASARREIGG GEAGAVIGGS AGASPPSTLT PDSRRVARPP
PIGAEVPDVT ATPARLLFFA PTRRAAPLEE MEAPAADAIM SPEEELDGYE PEPLGKRPAV
LPLLELVGES GNNTSTDGSL PSTPPPAEEE EDELYRQSLE IISRYLREQA TGAKDTKPMG
RSGATSRKAL ETLRRVGDGV QRNHETAFQG MLRKLDIKNE DDVKSLSRVM IHVFSDGVTN
WGRIVTLISF GAFVAKHLKT INQESCIEPL AESITDVLVR TKRDWLVKQR GWDGFVEFFH
VEDLEGGI
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against MCL1. It has been
selected for its ability to recognize MCL1 in immunohistochemical staining and
western blotting.Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant MCL1 (Arg6~Ile328)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Ovary tissue
Used in Western Blot, Sample:
Recombinant MCL1, Human
