Polyclonal Antibody Antibody Antibody Antibody to Osteoclast Osteoclast Osteoclast Osteoclast Associated Associated Associated Associated Receptor Receptor Receptor Receptor (OSCAR) (OSCAR) (OSCAR) (OSCAR)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

7th Edition (Revised in May, 2013)

[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: OSCAR, Human

Clonality: Clonality: Clonality: Clonality: Polyclonal

Host: Rabbit

Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG

Purification: Purification: Purification: Purification: Affinity Chromatography.破骨细胞关联受体(OSCAR)多克隆抗体

Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: Concentration: Concentration: Concentration: 200µg/mL

UOM: 100µg Sample: Recombinant OSCAR, Human

[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: Recombinant OSCAR (Asp19~Ser220) expressed in E.coli. USCN Accession Accession Accession Accession No.: rP92695Hu01

Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence

is listed below.

MGHHHHHHSG S- DI TPSVAIIVPP ASYHPKPWLG AQPATVVTPG VNVTLRCRAP

QPAWRFGLFK PGEIAPLLFR DVSSELAEFF LEEVTPAQGG IYRCCYRRPD WGPGVWSQPS

DVLELLVTEE LPRPSLVALP GPVVGPGANV SLRCAGRLRN MSFVLYREGV AAPLQYRHSA

QPWADFTLLG ARAPGTYSCY YHTPSAPYVL SQRSEVLVIS

[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against OSCAR. It has been

selected for its ability to recognize OSCAR in immunohistochemical staining and

western blotting.破骨细胞关联受体(OSCAR)多克隆抗体

[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS CONTENTS CONTENTS CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY QUALITY QUALITY QUALITY CONTROL ]

Content: Content: Content: Content: The quality control contains recombinant OSCAR (Asp19~Ser220)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemil umes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

[ STORAGE ]破骨细胞关联受体(OSCAR)多克隆抗体

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.