Polyclonal Antibody Antibody Antibody Antibody to Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2)

Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

7th Edition (Revised in May, 2013)

[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: DIO2, Rat

Clonality: Clonality: Clonality: Clonality: Polyclonal

Host: Rabbit

Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG

Purification: Purification: Purification: Purification: Affinity Chromatography.碘甲腺原氨酸脱碘酶Ⅱ(DIO2)多克隆抗体

Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: Concentration: Concentration: Concentration: 200µg/mL

UOM: 100µg Sample: Recombinant DIO2, Rat

[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: Recombinant DIO2 (Ser30~Phe259) expressed in E.coli. USCN Accession Accession Accession Accession No.: rP92903Ra01

Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and

MBP-tag and its sequence is listed below.

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI

IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK

DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK

DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK

VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL

GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE

ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ

GPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED

APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF

RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS

LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF

[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against DIO2. It has been

selected for its ability to recognize DIO2 in immunohistochemical staining and

western blotting.碘甲腺原氨酸脱碘酶Ⅱ(DIO2)多克隆抗体

[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS CONTENTS CONTENTS CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY QUALITY QUALITY QUALITY CONTROL ]

Content: Content: Content: Content: The quality control contains recombinant DIO2 (Ser30~Phe259)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemil umes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.碘甲腺原氨酸脱碘酶Ⅱ(DIO2)多克隆抗体