Ⅱ型胶原α1(COL2α1)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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标记物Ⅱ型胶原α1(COL2α1)多克隆抗体无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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Ⅱ型胶原α1(COL2α1)多克隆抗体
Antibody Antibody Antibody Antibody to Collagen Collagen Collagen Collagen Type II Alpha 1 (COL2a1) (COL2a1) (COL2a1) (COL2a1)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: COL2a1
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Organism: Organism: Organism: Organism: Human
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.Ⅱ型胶原α1(COL2α1)多克隆抗体
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Concentration: Concentration: Concentration: Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant Human COL2a1
[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human COL2a1 (Ser1386~Pro1467) expressed in
E.coli. USCN Accession Accession Accession Accession No.: P93194Hu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSGSEF- SQNIT YHCKNSIAYL DEAAGNLKKA LLIQGSNDVE IRAEGNSRFT
YTALKDGCTK HTGKWGKTVI EYRSQKTSRL PIIDIAP
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
Anti COL2a1 is a rabbit polyclonal antibody raised against human COL2a1. This
antibody has been selected for its ability to recognize human COL2a1 in
immunohistochemical staining and western blotting, non cross-reactive with other
members of the family.Ⅱ型胶原α1(COL2α1)多克隆抗体
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% Na3N,
50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant human
COL2a1(Ser1386~Pro1467) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, Na3N 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.Ⅱ型胶原α1(COL2α1)多克隆抗体
