转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体
Polyclonal Antibody
To Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: TRPM7, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant TRPM7 (Ile19~Gly179) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPF837Ra01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against TRPM7. It has been
selected for its ability to recognize TRPM7 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant TRPM7 (Ile19~Gly179)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
转化受体电位阳离子通道亚家族M成员7(TRPM7)多克隆抗体[ IMAGES ]
Used in Western Blot, Sample:
Recombinant TRPM7, Rat
Used in DAB staining on fromalin fixed
paraffin- embedded brain tissue
PAF837Ra01
Polyclonal Antibody
To Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Rat Serum
Lane2: Mouse Pancreas Tissue
Primary Ab: 1.5µg/mL Rabbit Anti-Rat TRPM7 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
