Nexn结合蛋白(NEXN)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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Nexn结合蛋白(NEXN)多克隆抗体标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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Nexn结合蛋白(NEXN)多克隆抗体
Polyclonal Antibody Antibody Antibody Antibody to Nexilin Nexilin Nexilin Nexilin (NEXN)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: NEXN, Human
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Concentration: Concentration: Concentration: Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant NEXN, Human
Nexn结合蛋白(NEXN)多克隆抗体[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant NEXN (Glu409~Val605) expressed in E.coli. USCN Accession Accession Accession Accession No.: P97548Hu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSG SEF- EQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS
KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK
VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST
AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKV
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against NEXN. It has been
selected for its ability to recognize NEXN in immunohistochemical staining and
western blotting.
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant NEXN (Glu409~Val605)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Nexn结合蛋白(NEXN)多克隆抗体[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
