毛状样蛋白1(COTL1)多克隆抗体
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来源多抗制备
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宿主兔
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效价-
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Ig类型IgG
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纯化方式抗原特异性亲和纯化
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毛状样蛋白1(COTL1)多克隆抗体标记物无标记物
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**原-
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缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
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性状液体
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浓度200ug/ml
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且适物种-
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应用WB, ICC, IHC-P, IHC-F, ELISA
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毛状样蛋白1(COTL1)多克隆抗体
Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: COTL1, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
毛状样蛋白1(COTL1)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant COTL1 (Met1~Glu142) with two N-terminal Tags,
His-tag and GST-tag expressed in E.coli. Accession No.: RPJ353Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against COTL1. It has been
selected for its ability to recognize COTL1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
毛状样蛋白1(COTL1)多克隆抗体[ QUALITY CONTROL ]
Content: The quality control contains recombinant COTL1 (Met1~Glu142)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant COTL1, Human
Used in DAB staining on fromalin fixed
paraffin- embedded testis tissue
PAJ353Hu01
Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
j10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Human Lung Tissue
Lane2: Hela Cell
Lane3: Mouse Placenta Tissue
Lane3: Mouse Kidney Tissue
Primary Ab: 1µg/mL Rabbit Anti-Human COTL1 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
