酰基羧酸水解酶(AOAH)多克隆抗体
Polyclonal Antibody to Acyloxyacyl Hydrolase (AOAH)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: AOAH, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant AOAH (Ser24~Gln300) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPJ634Hu01
酰基羧酸水解酶(AOAH)多克隆抗体[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against AOAH. It has been
selected for its ability to recognize AOAH in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant AOAH (Ser24~Gln300)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
酰基羧酸水解酶(AOAH)多克隆抗体[ IMAGES ]
Used in Western Blot, Sample:
Recombinant AOAH, Human
Used in DAB staining on fromalin fixed
paraffin- embedded pancreas tissue
PAJ634Hu01
Polyclonal Antibody to Acyloxyacyl Hydrolase (AOAH)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Human Serum
Lane2: Pig Intestine Tissue
Lane3: Pig Stomach Tissue
Lane4: Pig Spleen Tissue
Primary Ab: 2µg/mL Rabbit Anti-Human AOAH Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
