来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
组织蛋白酶A(CTSA)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
组织蛋白酶A(CTSA)多克隆抗体Polyclonal Antibody to Cathepsin A (CTSA)
Organism Species: Canis familiaris; Canine (Dog)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CTSA, Canine
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant CTSA, Canine
组织蛋白酶A(CTSA)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CTSA (Asp234~Leu494) expressed in E.coli. Accession No.: RPA348Ca01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DNSLVYF AYYHGLLGNR
LWSSLQTHCC SQNKCNFYDN TDPECVTNLQ EVSRIVGNSG LNIYNLYAPC AGGVPGHLRY
EKDTVVLHDL GNIFTRLPFK RVWHQALLRS DDRLRMDPPC TNTTAASTYLNNPYVRKALH
IPEQLPRWDM CNFLVNIQYR RLYQSMQSQY LKLLTTQKYR ILLYNGDVDM ACNFMGDEWF
VDSLNQKMEV QRRPWLVDYG DSGEQIAGFV KEFSHIAFLT IKGAGHMVPT DKPQAALTMF
SRFL
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CTSA. It has been
selected for its ability to recognize CTSA in immunohistochemical staining and
western blotting.
组织蛋白酶A(CTSA)多克隆抗体[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant CTSA (Asp234~Leu494)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.