丝裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)多克隆抗体
Polyclonal Antibody
To Mitogen Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: MAP4K5, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
丝裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant MAP4K5 (Thr613~His842) expressed in E.coli. Accession No.: RPB135Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTKIPDTK GCHKCCIVRN
PYTGHKYLCG ALQSGIVLLQ WYEPMQKFML IKHFDFPLPS PLNVFEMLVI PEQEYPMVCV
AISKGTESNQ VVQFETINLN SASSWFTEIG AGSQQLDSIH VTQLERDTVL VCLDKFVKIV
NLQGKLKSSK KLASELSFDF RIESVVCLQD SVLAFWKHGM QGKSFKSDEV TQEISDETRV
FRLLGSDRVV VLESRPTENP TAHSNLYILA GH
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against MAP4K5. It has been
selected for its ability to recognize MAP4K5 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
丝裂原激活蛋白激酶激酶激酶激酶5(MAP4K5)多克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant MAP4K5 (Thr613~His842)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Kidney tissue
Used in Western Blot, Sample:
Recombinant MAP4K5, Human
