来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
肌动蛋白β(ACTb)多克隆抗体标记物无标记物
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
肌动蛋白β(ACTb)多克隆抗体Polyclonal Antibody to Actin Beta (ACTb)
Organism Species: Multi-species
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200µg/mL
UOM: 100µg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPB340Mi01-Recombinant Actin Beta (ACTb)
肌动蛋白β(ACTb)多克隆抗体[ ORGANISM SPECIES MORE ]
React with: human, mouse, rat, cavia, ovine, canine, porcine, equine, gallus, and
bovine;
Other species have not been detected. [ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
肌动蛋白β(ACTb)多克隆抗体[ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ]
Content: The quality control contains recombinant ACTb disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A
Figure1. Western Blot
A. Sample: Recombinant ACTb
B.Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Human MCF-7 Cells;
Lane4: Human BXPC-3 Cells; Lane5: Human A875 Cells; Lane6: Human A431 Cells;
Lane7: Human Raji Cells; Lane8: Human RAW264.7 Cells; Lane9: Human PC-3 Cells;
Lane10: Human SGC7901 Cells; Lane11: Human HL60 Cells; Lane12: Human A-375 Cells;
Lane13: Human Jurkat Cells; Lane14: Human SKOV3 Cells;
Primary Ab: µg/mL Rabbit Anti-Human ACTb Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)
B