来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
含LIM调宁蛋白同源域蛋白1(LIMCH1)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
含LIM调宁蛋白同源域蛋白1(LIMCH1)多克隆抗体Polyclonal Antibody
To LIM And CalPonin Homology Domains Containing Protein 1 (LIMCH1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: LIMCH1, Mouse
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
含LIM调宁蛋白同源域蛋白1(LIMCH1)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant LIMCH1 (Glu21~Asn125) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPR961Mu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against LIMCH1. It has been
selected for its ability to recognize LIMCH1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
含LIM调宁蛋白同源域蛋白1(LIMCH1)多克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant LIMCH1 (Glu21~Asn125)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant LIMCH1, Mouse
Used in DAB staining on fromalin fixed
paraffin- embedded brain tissue
PAR961Mu01
Polyclonal Antibody
To LIM And CalPonin Homology Domains Containing Protein 1 (LIMCH1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample: Mouse Lung Tissue
Primary Ab: 1µg/mL Rabbit Anti-Mouse LIMCH1 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)