来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
多萜醇二磷酸寡糖蛋白环糊精糖基转移酶(DDOST)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
多萜醇二磷酸寡糖蛋白环糊精糖基转移酶(DDOST)多克隆抗体Polyclonal Antibody
To Dolichyl Diphosphooligosaccharide Protein Glycosyltransferase (DDOST)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: DDOST, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
多萜醇二磷酸寡糖蛋白环糊精糖基转移酶(DDOST)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant DDOST (Ser43~Pro427) expressed in E.coli. Accession No.: RP279Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSG S-SGPRTLVL LDNLNVRETH SLFFRSLKDR GFELTFKTAD DPSLSLIKYG
EFLYDNLIIF SPSVEDFGGN INVETISAFI DGGGSVLVAA SSDIGDPLRE LGSECGIEFD
EEKTAVIDHH NYDISDLGQH TLIVADTENL LKAPTIVGKS SLNPILFRGV GMVADPDNPL
VLDILTGSST SYSFFPDKPI TQYPHAVGKN TLLIAGLQAR NNARVIFSGS LDFFSDSFFN
SAVQKAA PGS QRYS QT GNYE LAVA LS RWV F KEEGV L RVGP VS H HRV GE TA
PPNAYTVTDL VEYSIVIQQL SNGKWVPFDG DDIQLEFVRI DPFVRTFLKK KGGKYSVQFK
LPDVYGVFQF KVDYNRLGYT HLYSSTQVSV RPLQHTQYER FIPSAYP
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against DDOST. It has been
selected for its ability to recognize DDOST in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
多萜醇二磷酸寡糖蛋白环糊精糖基转移酶(DDOST)多克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant DDOST (Ser43~Pro427)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin- embedded stomach tissue
Used in Western Blot ,Sample:
Recombinant DDOST,Human