来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
补体成分3a(C3a)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
补体成分3a(C3a)多克隆抗体Polyclonal Antibody Antibody Antibody Antibody to Complement Complement Complement Complement Component Component Component Component 3a (C3a)
Organism: Organism: Organism: Organism: Mus musculus musculus musculus musculus (Mouse) (Mouse) (Mouse) (Mouse)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
6th Edition (Revised in March, 2013)
补体成分3a(C3a)多克隆抗体[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: C3a, Mouse
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F
Concentration: Concentration: Concentration: Concentration: 200µg/mL
UOM: 100µg Sample: Recombinant C3a, Mouse
[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant C3a (Glu1314~Asn1663) expressed in E.coli. USCN Accession Accession Accession Accession No.: P90387Mu01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MG H HH H HHSGSEF -EN G N L L R SEE TK Q NEAF SLTAKGK GR G TLSVVAVY HA
KLKSKVTCKK FDLRVSIRPA PETAKKPEEA KNTMFLEICT KYLGDVDATM SILDISMMTG
FAPDTKDLEL LASGVDRYIS KYEMNKAFSN KNTLIIYLEK ISHTEEDCLT FKVHQYFNVG
LIQPGSVKVY SYYNLEESCT RFYHPEKDDG MLSKLCHSEM CRCAEENCFM QQSQEKINLN
VRLDKACEPG VDYVYKTELT NIELLDDFDE YTMTIQQVIK SGSDEVQAGQ QRKFISHIKC
RNA LK LQKGK KY LMWG LSS D LWGEKP NTSY IIGKD TW VEH WPEAEEC Q DQ
KYQKQCEELG AFTESMVVYG CPN
补体成分3a(C3a)多克隆抗体[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against C3a. It has been
selected for its ability to recognize C3a in immunohistochemical staining and
western blotting.
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant C3a (Glu1314~Asn1663)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.