来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
PP2A癌症抑制抑制因子(CIP2A)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
PP2A癌症抑制抑制因子(CIP2A)多克隆抗体Polyclonal Antibody to Cancerous Inhibitor Of PP2A (CIP2A)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CIP2A, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
PP2A癌症抑制抑制因子(CIP2A)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CIP2A (Asn230~Glu560) expressed in E.coli. Accession No.: RPR982Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N IHQTFQLIFN
ILINGDGTLT RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL
ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ PLDGSENCSV
LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT EQNLDEALTR KKCERIAKAI
EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE QQFTYGKIDL GFGTKVADSE LCKLAADVIL
KTLDLINKLK PLVPGMEVSF YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD
FPALVLGESI AANNAYRQQE
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CIP2A. It has been
selected for its ability to recognize CIP2A in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
PP2A癌症抑制抑制因子(CIP2A)多克隆抗体[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant CIP2A (Asn230~Glu560)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
Used in DAB staining on fromalin fixed
paraffin- embedded Testis tissue
Used in Western Blot, Sample:
Recombinant CIP2A, Human
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.