来源多抗制备
宿主兔
效价-
Ig类型IgG
纯化方式抗原特异性亲和纯化
ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体标记物无标记物
**原-
缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
性状液体
浓度200ug/ml
且适物种-
应用WB, ICC, IHC-P, IHC-F, ELISA
ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体Polyclonal Antibody to ATP Dependent DNA ligase I (LIG1)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: LIG1, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200µg/mL
UOM: 100µg
ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant LIG1 (Lys568~Phe875) expressed in E.coli. Accession No.: RPB269Ra01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSG LVPRGSH MASMTGGQQM GRGSEF - KYY GQRAQIHVLE
GGEVKIFSRN QEDNSGKYPD IISRIPKIKH PSVTSFILDT EAVAWDREKK QIQPFQVLTT
RKRKEVDASE IQVQVCLYAF DLIYLNGESL ARQPLSRRRQ LLRENFVETE GEFVFATSLD
TKDIEQIAEF LEQSVKDSCE GLMVKTLDVD ATYEIAKRSH NWLKLKKDYL EGVGDTLDLV
VIGAYLGRGK RPGRYGGFLL AAYDEESEEL AAICKLGTGF SDEELEEHHQ NMQALLLPTP
RPYVRIDGAV APNHWLDPSI VWEVKCADLT LSPIYRAARG MVDKEKGISL RFPRF
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against LIG1. It has been
selected for its ability to recognize LIG1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
ATP依赖性DNA连接酶Ⅰ(LIG1)多克隆抗体[ QUALITY CONTROL ]
Content: The quality control contains recombinant LIG1 (Lys568~Phe875)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Brain tissue
Used in Western Blot, Sample:
Recombinant LIG1, Rat